Determination of sulfobromophthalein in serum.

Studies on hepatic sulfobromophthalein removal during infusion.

Experiments were performed with the infusion of sulfobromophthalein (BSP) for brief periods (15 mm.) in man, following an initial massive dose of dye at the beginning of the experiment. Infusion was such as to ensure a continuous increase in the plasma BSP level. In these conditions, BSP removal due to the storage capacity of the liver was studied. The rate of removal was found to depend on the...

Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes.

When [14C]oleate-bovine serum albumin complexes were incubated in vitro with rat liver plasma membranes (LPM), specific, saturable binding of oleate to the membranes was observed. Maximal heat-sensitive (i.e., specific) binding was 3.2 nmol/mg of membrane protein. Oleate-agarose affinity chromatography of Triton X-100-solubilized LPM was used to isolate a single 40-kDa protein with high affinit...

Studies of Sulfobromophthalein Sodium (bsp) Metabolism in Man. Ii. the Effect of Artificially Induced Fever, Norethandrolone (nilevar), and Iopanoic Acid (telapaque).

In vitro evaluation of heparinized Cuprophan hemodialysis membranes.

Cuprophan hemodialysis membranes can be heparinized using N,N'-carbonyldiimidazole (CDI) as a coupling agent. In this study, the characteristics of heparinized Cuprophan membranes have been evaluated. After immobilization, heparin partially retained its biologic activity. An anticoagulant activity of 12.4 +/- 4.2 mU/cm2 was measured using a thrombin inactivation assay. Immobilized heparin also ...

Identification of metabolites of sulfobromophthalein.

Sulfobromophthalein (BSP) is normally cleared from the blood by the liver and excreted into the bile. Brauer, Krebs and Pessotti (1) originally reported that part of the pigment appearing in dog bile was chromatographically different from the injected dye. Our studies using differenlt chromatographic techniques indicate that in man the bulk of the recovered pigments (65 to 75 per cent, accordin...